Polyadenylic Acid (5′) Potassium Salt is a polymer composed of multiple adenosine units. It is a white to pale yellow powdery solid that is soluble in water and insoluble in organic solvents. It is typically stored at -20 ° C. This compound has important biological functions within cells, capable of forming double strands with RNA or DNA and participating in the regulation of gene expression.
Polyadenylic Acid (5′) Potassium Salt has a wide range of applications in the fields of biochemistry and molecular biology. The following are its main application areas:

Nucleic acid extraction and purification:
Polyadenylic Acid (5′) Potassium Salt is used as a carrier RNA (Poly A), mainly for extracting DNA/RNA, short nucleic acids (<200bp) or small RNAs from trace samples such as virus samples, to help improve the recovery or extraction efficiency of DNA and RNA. It can improve the binding and elution efficiency of trace DNA/RNA samples during the purification process, reducing the probability of degradation by residual nucleases that may exist. This application is particularly important in fields such as virology research, genomics, and transcriptomics, as these fields often require the handling of small or valuable samples.

MRNA vaccines and drug development:
The dynamic changes of Polyadenylic Acid (5 ‘) Potassium Salt are crucial for regulating gene expression, which is particularly important in the development of mRNA vaccines and drugs. By regulating the length and structure of poly (A) tails, the stability and translation efficiency of mRNA can be affected, thereby affecting the efficacy of vaccines and drugs. In the design of mRNA vaccines, appropriate poly (A) tail length and structure can enhance the stability and immunogenicity of the vaccine, which is crucial for its effectiveness and safety.

• Experimental tools:
Polyadenylic Acid (5′) Potassium Salt is also used as a reagent in laboratory research for cell genetics and biosynthesis studies. For example, it is used as a carrier for quantitative precipitation of DNA and RNA, as well as for applications in droplet digital PCR (ddPCR) analysis. In these applications, Polyadenylic Acid (5′) Potassium Salt provides an effective tool to improve the accuracy and efficiency of experiments.

• Enzyme activity inhibition:
Polyadenylic Acid (5′) Potassium Salt can inhibit the exonuclease activity of Exo1, which is very useful in studying the function and mechanism of nucleases. By using Polyadenylic Acid (5′) Potassium Salt, researchers can more accurately control and study the activity of nucleases, which is crucial for understanding DNA repair, recombination, and other nucleic acid metabolism processes.

In summary, Polyadenylic Acid (5′) Potassium Salt has important applications in various fields such as biochemical research, molecular biology techniques, gene expression regulation, epigenetics, and biopharmaceutical development. By delving into the properties and functions of Polyadenylic Acid (5′) Potassium Salt, we can better understand the molecular mechanisms of life and develop more effective biotechnology and therapeutic methods.